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Vopr Med Khim. 2002 May-Jun;48(3):286-92.
[Nitric oxide generation by human peripheral blood platelets in the norm and after thoracic and abdominal wounds]

[Article in Russian]

Abakumov MM, Golikov PP, Nikolaeva NIu, Klychnikova EV, Stotskaia TV, Davydov BV, Matveev SB.

Sklifosovsky Research Institute for Emergency Medicine, 129010, Moscow, B. Sukharevskaya Square, 3.

Nitric oxide production by the human peripheral blood platelets in normal condition and after thoracic and abdominal wounds was investigated. The platelets were obtained from blood by differential centrifuging. The nitric oxide production by platelets (0.5 x 10(8)) was studied in RPMI 1640 medium containing bovine fetal serum, glutamine, and antibiotics, during incubation at 37 degrees C for 15 hours. Nitric oxide level was determined by Griss reaction. A large-scale absolute production of nitric oxide by platelets was revealed both in normal condition (7.70 +/- 0.55 mumol/l), and after thoracic and abdominal wounds (11.45 +/- 0.84 mumol/l). Severe trauma course is associated with hyperproduction of nitric oxide possessing multiple damaging effects, including the anti-thrombogenic one, which should be taken into account in clinical practice.


online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12243087&dopt=Abstract







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