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Am J Vet Res. 2000 Nov;61(11):1451-5.
Methicillin-resistant coagulase-negative staphylococci isolated from healthy horses in Japan.

Yasuda R, Kawano J, Onda H, Takagi M, Shimizu A, Anzai T.

Department of Microbiology and Immunology, Faculty of Agriculture, Kobe University, Japan.

OBJECTIVE: To determine patterns of methicillin-resistant staphylococci isolated from apparently healthy horses. SAMPLE POPULATION: 44 horses from 8 riding clubs in Japan. PROCEDURE: Methicill in-resistant staphylococci were isolated from the skin or nares, using a selective medium containing a beta-(symboric) lactam antibiotic, ceftizoxime. Clonality of isolates was determined by use of pulsed-field gel electrophoresis. Detection of mecA, mecl, and mecR1 genes was accomplished by use of polymerase chain reactions. RESULT: Of the 44 horses, 13 (29.5%) yielded 15 isolates of methicillin-resistant staphylococci. The 15 isolates were identified as 6 species (Staphylococcus epidermidis, S lentus, S saprophyticus, S xylosus, S sciuri, and S haemolyticus). However, methicillin-resistant S aureus was seldom isolated. Each isolate contained the mecA gene and had a high resistance to beta-lactam antibiotics. Some isolates also were resistant to other antibiotics such as erythromycin and kanamycin. CONCLUSIONS AND CLINICAL RELEVANCE: Methicillin-resistant coagulase-negative staphylococci that were highly resistant to various antibiotics were isolated from apparently healthy horses in Japan. These organisms must be considered a potential threat to horses and veterinarians who care for them.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11108196&dopt=Abstract

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