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Forensic Sci Int. 2002 Apr 18;126(2):118-22.
Immunohistochemical detection of fibronectin and tenascin in incised human skin injuries.
Ortiz-Rey JA, Suarez-Penaranda JM, Da Silva EA, Munoz JI, San Miguel-Fraile P, De la Fuente-Buceta A, Concheiro-Carro L.
POVISA (Policlinico de Vigo SA), Vigo, Spain.
Immunohistochemical detection of molecules involved in inflammatory reaction can be useful for the diagnosis of vitality in skin wounds. We studied the expression of fibronectin (FN) and tenascin (TN) in 58 human skin wounds (48 vital and 10 postmortem). The age of vital injuries ranged from 3 min to 8 h and postmortem specimens were collected after a postinfliction interval of 15-180 min. One hundred thirty-seven formalin-fixed paraffin-embedded sections (mean: 2.3 sections per case) were stained with each of two monoclonal antibodies against FN and TN using the streptABC technique. A reticular staining for FN in wound edge and dermis was observed in 50% of vital specimens versus 0% in postmortem cases. Immunoreactivity was reduced in 10 autolysed cases. FN positivity exclusively at the injury margin was observed in 39.4% of vital wounds and 10% of postmortem cases. TN was negative in all specimens. Vital and postmortem hemorrhage areas showed positivity for FN and TN. Due to its low sensitivity, immunohistochemical analysis of FN is useful for determining vitality only in a minority of cases. Different factors in everyday practice, including autolysis and technical problems often produce false negative reactions with the result that FN cannot be regarded as a reliable parameter of vitality. Positive reactions (network staining) are more valuable than negativity but are not pathognomonic. Both vital and postmortem hemorrhages show an enhanced positivity for FN and TN, thus impeding the diagnosis.
Online source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12084487&dopt=Abstract hemorrhage
Zentralbl Chir. 2002 Apr;127(4):329-31.
[Acute hemorrhage from Meckel's diverticulum--Laparotomy or laparoscopy?]
[Article in German]
Knoop M, Vorwerk T, Friedrichs KS.
Klinik fur Allgemein- und Viszeralchirurgie, Johanniter Krankenhaus der Altmark, Stendal, Akademisches Lehrkrankenhaus der Otto-von-Guericke Universitat Magdeburg, Germany. Mdrknoool.com
Meckel's diverticulum is the most common congenital malformation of the gastrointestinal tract with a potential risk to develop complications such as obstruction, diverticulitis or intussusception. Lower gastrointestinal bleeding due to ulceration of heterotopic gastric tissue of the diverticulum is a known phenomenon in children and young adults. We present two cases of a 15-year-old girl and a 20-year-old man that revealed a massive lower gastrointestinal hemorrhage of unknown origin. In this emergency situation laparotomy was performed in combination with lower endoscopy as rendezvous manouver. In both cases a Meckel's diverticulum with peptic ulceration was the source of hemorrhage, in one case the bleeding was active and visible. After resection of a short small bowel segment and end-to-end anastomosis the postoperative course was uneventful. We prefer in the case of lower gastrointestinal hemorrhage with hemodynamic instability laparotomy with intraoperative endoscopy instead of laparoscopy.
Online source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12085287&dopt=Abstract hemorrhage
Nefrologia. 2002;22(2):190-5.
[Simultaneous presence of antibodies against the glomerular basement membrane and anti-myeloperoxidase antibodies in 2 patients with rapidly progressive glomerulonephritis]
[Article in Spanish]
Diaz Rodriguez C, Costero O, Torre A, De Alvaro F, Gil F, Picazo ML, Martinez-Ara J.
Servicio de Nefrologia, Departamento de Anatomia Patologica, Hospital Universitario La Paz Madrid. cdiazulp.insalud.es
We report two patients with rapidly progressive glomerulonephritis without alveolar hemorrhage. Renal biopsy showed extracapillary glomerulonephritis with linear deposits of immunoglobulin G. Serologically anti-glomerular basement membrane antibodies (Ac AMBG) and ANCA anti-myeloperoxidase were present. All patients were treated with steroids, cyclophosphamide and plasma exchange. One patient needed dialysis, and other one died from a renal biopsy complication. We discuss the epidemiologic, pathogenic and prognostic aspects of this association.
Online source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12085420&dopt=Abstract hemorrhage
Optom Vis Sci. 2002 Jun;79(6):353-62.
Ocular and systemic findings and their correlation with hemodynamically significant carotid artery stenosis: a retrospective study.
Lyons-Wait VA, Anderson SF, Townsend JC, De Land P.
VA Greater Los Angeles Healthcare System, Sepulveda Ambulatory Care Center and Nursing Home, California, USA.
BACKGROUND: Optometrists often encounter patients with ocular signs and/or symptoms suggestive of carotid artery disease, but criteria for eye care practitioners concerning when to order carotid studies are not well established. A retrospective study in an optometry clinic was performed to determine if certain ocular findings and associated systemic risk factors were associated with hemodynamically significant carotid artery stenosis (HSCAS). METHODS: A retrospective analysis was performed on all patients examined in the optometry clinic at the Sepulveda Ambulatory Care Center from January 1, 1998 through December 31, 1999 to identify all patients who had carotid studies ordered. Charts were then reviewed to determine the ocular finding that prompted the carotid study. Statistical analysis using an odds-ratio was performed to determine whether any ocular sign/symptom or systemic risk factor was associated with HSCAS. RESULTS: Of 3822 patients, 48 (1.26%) had carotid studies ordered. Eight (17%) had HSCAS of >50% and symptomatic patients were 1.6 times more likely to have HSCAS than asymptomatic patients. Patients with HSCAS were 1.8 times more likely to have retinal vascular occlusions, 1.9 times more likely to have normotensive glaucoma, 2.4 times more likely to have peripheral retinal hemorrhages, and 2.6 times more likely to be smokers, although none of these factors were found to be a statistically significant indicator of HSCAS. However, the number of systemic diseases tended to be greater for the HSCAS patients compared with the non-HSCAS patients (HSCAS median = 3.5 and the non-HSCAS median = 2, p = 0.049). CONCLUSION: Although no single ocular or systemic risk factor was found to be a statistically significant indicator of HSCAS, the number of systemic disease risk factors tended to be greater for the HSCAS patients compared with the non-HSCAS patients (p = 0.049). An extensive literature review was also performed to help establish guidelines for when to order carotid studies on the basis of ocular signs and/or symptoms as well as the presence of additive associated systemic risk factors.
Online source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12086301&dopt=Abstract hemorrhage
Contemp Top Lab Anim Sci. 1999 May;38(3):80-83.
High Mortality in Zebrafish (Danio rerio).
Pullium JK, Dillehay DL, Webb S.
Division of Animal Resources, Emory University, School of Medicine, Atlanta, GA 30322.
A group of 100 adult zebrafish were housed in a new system at a stocking density of 20 fish per tank. Four weeks after arrival, 15 fish presented with petechial hemorrhages and ulceration on the surfaces of the skin. Samples of the fish were collected for histopathology, fungal culture, and bacterial culture and sensitivity. Water samples were analyzed for pH, ammonia, nitrite, and submitted for bacterial and fungal culture. Histologically, the epidermis had multiple areas of ulceration and mononuclear cell infiltrate. Gram-positive bacteria were observed beneath the surface of the skin and surrounding the outer aspect of the spinal cord. Both Aeromonas hydrophila and A. sobria were isolated from the affected fish, and a diagnosis of motile aeromonad septicemia (MAS) was made. Water from the tanks had a nitrite level of 1-5 ppm, a toxic concentration that indicated poor water quality. Because the housing system had been seeded with Nitrobacter spp. and Nitrosomonas spp. only 2 weeks prior to the arrival of the fish, a lack of colonizing nitrifying bacteria was deemed to be the cause of the high nitrite level, which, along with over-crowding, stressed the fish and increased their susceptibility to MAS. No further cases of septicemia were observed once the nitrite level and stocking density were reduced.
Online source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12086431&dopt=Abstract hemorrhage [PubMed - as supplied by publisher]
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